Liquid Biopsy Stool DNA qPCR Mix
PCR inhibitors found in stool specimens, such as bile salts, polysaccharides, hematin and catabolic substances, have posed a big challenge to developing assays.
PCR inhibitors found in stool specimens, such as bile salts, polysaccharides, hematin and catabolic substances, have posed a big challenge to developing assays.
Highly sensitive detection of target DNA from whole blood, plasma, and serum samples
X-GAL is a chromogenic substrate for ß-Galactosidase used to differentiate recombinants from nonrecombinants in cloning experiments using vectors containing the lacZ or lacZ α-peptide gene.
Uracil DNA Glycosylase (UDG) catalyzes the release of uracil from uracil-containing single-stranded or double-stranded DNA, but not from RNA or oligonucleotides (6 or fewer bases).UDG is active over a broad pH range with an optimum at pH 8.0, does not require a divalent cation, and is inhibited by high ionic strength (>200 mM).Meridian UDG is purified to SDS-PAGE purity and is free of endonucleases, exonucleases, nickases and RNases. Source: Recombinant E. coli strain carrying the over expressed gene of Uracil DNA Glycosylase from E. coli.
Tetro™ Reverse Transcriptase is a highly sensitive, high stability MMLV reverse transcriptase. Tetro Reverse Transcriptase is optimized for reverse transcription reactions using a wide range of total RNA amounts, such that long and low abundance cDNAs can be detected by amplification after cDNA synthesis.
Tetro Reverse Transcriptase is suitable for first-strand cDNA synthesis, cDNA library construction, and the production of templates for RT-PCR analysis of gene expression.
Tetro™ cDNA Synthesis Kit contains all the necessary components to generate cDNA from an RNA template. The generated cDNA is suitable for PCR with gene-specific primers or for other downstream applications. The kit contains MMLV Reverse Transcriptase and is suitable for first strand cDNA synthesis, cDNA library construction and the production of templates for PCR amplification.
Outstanding assay reproducibility and sensitivity for both DNA and cDNA templates, under standard conditions.
Outstanding assay reproducibility and sensitivity for both DNA and cDNA templates, under standard conditions.
Outstanding assay reproducibility and sensitivity for both DNA and cDNA templates, under standard conditions.
Outstanding assay reproducibility and sensitivity for both DNA and cDNA templates, under standard conditions.
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