Meridian Bioscience (Bioline)

Uracil DNA Glycosylase

Uracil DNA Glycosylase (UDG) catalyzes the release of uracil from uracil-containing single-stranded or double-stranded DNA, but not from RNA or oligonucleotides (6 or fewer bases).UDG is active over a broad pH range with an optimum at pH 8.0, does not require a divalent cation, and is inhibited by high ionic strength (>200 mM).Meridian UDG is purified to SDS-PAGE purity and is free of endonucleases, exonucleases, nickases and RNases. Source: Recombinant E. coli strain carrying the over expressed gene of Uracil DNA Glycosylase from E. coli.


• Removes uracils from DNA – causes fragmentation of DNA and stops amplification in a PCR

• Easy-to-use – novel, sensitive mutant is irreversibly inactive after heating

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