NEXome Plus Panel v1.0 is an enhanced whole exome capture Panel. It extends the target regions to SNP skeleton of whole genome, intronic regions related to common gene fusion in solid tumors and classical microsatellite loci on the basis of NEXome Core Panel, which spans a 43.3 Mb region of human genome.
Based on the 1000 Genomes database, we mainly select >9,000 loci with high MAF values and high heterozygosity in the Chinese population, covering the entire human genome at approximately 300 Kb intervals. It provides richer and more evenly distributed information on the genomic composition based on the whole exome, and can be used for detection of CNVs and loss of heterozygosity.
Contains intron regions and non-coding regions related to common fusions and can be used to analyze fusion loci in solid tumors.
| ALK
intron 18 – 19 |
BCL2
3’UTR |
BCR
intron 8, 13 – 14 |
BRAF
intron 7 – 10 |
BRCA1
intron 2, 7 – 8, 12, 16, 19 – 20 |
BRCA2
intron 2 |
CD74
intron 6 – 8 |
EGFR
intron 7, 15, 24 – 27 |
ETV4
intron 5 – 6 |
ETV5
intron 6 – 7 |
ETV6
intron 5 – 6 |
| EWSR1
intron 6 – 13 |
EZR
intron 9 – 12 |
FGFR1
intron 1, 5, 17 |
FGFR2
intron 1, 17 |
FGFR3
intron 17 |
FLI1
intron 3 – 8 |
KIT
intron 16 |
KMT2A
intron 6 – 11 |
MET
intron 1, 14 |
MSH2
intron 5 |
MYB
intron 14 |
| MYC
intron 1 |
NOTCH2
intron 26 |
NTRK1
intron 8 – 10 |
NTRK2
intron 12, 15 |
NTRK3
intron 13 – 14 |
NUTM1
intron 1 |
PDGFB
intron 1 |
PDGFRA
intron 7, 9, 11 |
RAF1
intron 4 – 9 |
RARA
intron 2 |
RET
intron 7 – 11 |
| ROS1
intron 31 – 35 |
RSPO2
Upstream, 5’UTR, exon 1 – 2, intron 1 |
SDC4
intron 2 |
SLC34A2
intron 4 |
TMPRSS2
intron 1 – 3 |
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The NEXome Core Panel itself covers a large number of microsatellite loci, and the addition of 15 optimally designed MSI classical loci facilitates the comparison between different microsatellite instability analytical methods as well as the optimization and calibration of the analytical process.
| BAT-25 | BAT-26 | BAT-40 | BAT-RII | NR-21 | NR-22 | NR-24 | NR-27 | MONO-27 | D2S123 | D5S346 | D17S261 | D17S520 | D17S250 | D18S34 |
Capture Performance
Figure 1. Capture performance of NEXome Plus Panel v1.0 at 1-plex and 12-plex levels. Libraries were prepared using the NadPrep DNA Universal Library Preparation Kit (for Illumina®). NEXome Plus Panel v1.0 were used to complete hybridization capture. Sequencing platform: Illumina HiSeq X Ten, PE150. The BWA was used for alignment to the reference genome hg38, and on-target rate was calculated according to reads number.
Note：The samples: Human Male Genomic DNA (Promega-Male, G1471) and Human Female Genomic DNA (Promega-Female, G1521).
Figure 2. Capture performance of NEXome Plus Panel v1.0 in different gDNA libraries.Libraries were prepared using the NadPrep DNA Universal Library Construction Kit (for Illumina®). NEXome Plus Panel v1.0 (12 plex) were used to complete hybridization capture. Ssequencing platform: Illumina HiSeq X Ten with PE150. The BWA was used for alignment to the reference genome hg38, and on-target rate was calculated according to reads number.A. Mappability and on-target rate;B.Target covered;C.Coverage uniformity and consistency;D.GC bias.
| Catalog# | Color of Tube Cap | Product | Volume | Package/Storage |
| 1001861 |  |
NEXome Plus Panel v1.0, 96 rxn | 415 μL | –20℃ |
| 1001862 | |
NEXome Plus Panel v1.0, 16 rxn | 70 μL | –20℃ |
| Product | Catalog# |
| NEXome Plus Panel v1.0, 16 rxn | 1001862 |
| NEXome Plus Panel v1.0, 96 rxn | 1001861 |
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