NanOnco Plus Panel v3.0 targets the full coding regions from 620 genes (HLA genes included) of interest in solid tumor studies, a series of intron regions related to common fusions, classical microsatellite sequences, and chemotherapy-associated polymorphic loci. This panel involves a total number of 637 genes, covering an approximate 2.4 Mb target region of the genome. It supports the enrichment of multiple variants including base substitution, insertion/deletion, gene rearrangement, gene amplification, microsatellite instability and so on.
Capture performance
Multiple variant analysis
Figure 2. Consistency of the allele frequencies in NanOnco Plus Panel v3.0 capture data with the nominal frequencies of the standardsLibraries were prepared using NadPrep EZ DNA Library Preparation Kit coupled with NadPrep Universal Stubby Adapter (UDI), and sequenced on Illumina Novaseq 6000 with PE150. Variant analysis were performed using Vardict.
Note：Samples were Onco Multiplex gDNA (LDTBIO, custom standards) with an average sequencing depth of 3,518x.
Table 1. Detection of other variant types using NanOnco Plus Panel v3.0.
Libraries were prepared using NadPrep EZ DNA Library Prepation Kit coupled with NadPrep UDI Adapter (for Illumina®), and sequenced on Illumina Novaseq 6000 with PE150. Variant analysis were performed using Vardict, Delly and CNVkit. The average sequencing depth of GW-OGTM001 and GW-OGTM005 is 966.71x and 1,126.04x, respectively.
Note：Note: The sample was Onco Structural Multplex 5% gDNA (GeneWell, GW-OGTM001); the reference was Onco SNV Wildtype gDNA (GeneWell, GW-OGTM005).
| Catalog# | Color of Tube Cap | 称Product | Volume | Package/Storage |
| 1001112F |  |
NanOnco Plus Panel v3.0, 16 rxn | 70 μL | –20℃ |
| 1001111F | |
NanOnco Plus Panel v3.0, 96 rxn | 415 μL | –20℃ |
| Product | Catalog# |
| NanOnco Plus Panel v3.0, 16 rxn | 1001112F |
| NanOnco Plus Panel v3.0, 96 rxn | 1001111F |
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