NadPrep EZ DNA Library Preparation Kit v2 is designed for preparation of high-quality sequencing libraries from double-stranded DNA (dsDNA) on MGI and Illumina platforms. To simplify the experimental process, multiple processes were applied in one single step, including the fragmentation, end repair and adapter ligation. This A-T ligation-based kit applies to the whole genome sequencing with DNA input ranging from 5 to 500 ng, and is compatible with hybridization capture based targeted sequencing.
High Efficiency and Stability
Fig 1. Library yield of NadPrep EZ DNA Library Preparation Kit v2 for gDNA samples with different input amounts.The libraries were prepared by using NadPrep EZ DNA Library Preparation Module v2 (NadPrep Enfrag v2) coupled with A. NadPrep Universal Stubby Adapter (UDI) Module and NadPrep UDI Adapter Module (for Illumina®), and B. NadPrep Universal Adapter (SI) Module (for MGI) and NadPrep Universal Adapter (MDI) Module (for MGI) respectively, with 25 min of enzymatic digestion and recommended PCR cycles.
Compatible with Different Types of Samples
Fig 2. Library yield of NadPrep EZ DNA Library Preparation Kit v2 for different types of samples. The libraries were prepared by using NadPrep EZ DNA Library Preparation Module v2 coupled with A. NadPrep UDI Adapter Module (for Illumina®) and B. NadPrep Universal Adapter (MDI) Module (for MGI) respectively, with enzymatic digestion duration and PCR cycles as protocol.
Note:The grading standards by electrophoresis are as follows: gDNA: one bright and non-trailing stripe with about 15 kb in size. FFPE B+: one clear stripe with slight diffusion and with about 15 kb in size. FFPE B: one indistinct stripe with about 15 kb in size, with medium diffusion. FFPE C: multiple stripes ranging from 200 bp to 2,500 bp, with severe diffusion. FFPE D: multiple stripes ranging from 250 bp to 1,000 bp, with severe diffusion.
Flexible and Controllable Fragmentation
Fig 3. Size distribution of libraries prepared with different types of samples under different enzymatic digestion conditions.A.he libraries were prepared from 250 ng of gDNA sample with different grades by using NadPrep EZ DNA Library Preparation Module v2 coupled with NadPrep UDI Adapter Module (for Illumina®), with enzymatic digestion at different times as protocol, so as to obtain DNA fragments of different lengths.;B. The libraries were prepared from 50 ng of FFPE DNA samples of different grades, with enzymatic digestion at different times as protocol, so as to obtain DNA fragments of 250 bp.
Low GC Bias
Fig 4. Low GC bias in metagenomic library preparation using NadPrep EZ DNA Library Preparation Kit v2. After comparison of different library preparation schemes by using microbial community DNA standard (Zymo Research, D6306), the libraries prepared by using NadPrep EZ DNA Library Preparation Kit v2 show no significant difference in GC bias with those prepared by using sonication-based library preparation kits.
Note:NadPrep® Sonication: NadPrep®Both NadPrep Sonication: NadPrep DNA Universal Library Preparation Module (for Illumina®) and NadPrep Enfrag v2: NadPrep EZ DNA Library Preparation Kit V2 can be used along with NadPrep UDI Adapter Module (for Illumina®) for library preparation. (for Illumina®);
Low Background Noise Introduced
Fig 5. The abnormal reads ratio of libraries prepared with different types of samples by using different library preparation kits. The libraries were prepared with gDNA and different grades of FFPE DNA samples (50 ng each) with library preparation strategies, including NadPrep Sonication, NadPrep Enfrag v2, Vendor Q *, Vendor K* and Vendor V* enzymatic fragmentation library preparation kits. The multi-hybridization reaction was initiated with 500 ng of each library with the same library preparation scheme. The capture was performed by using NanOnco Plus Panel v2.0, with calculation of total reads. A. Library yield and the abnormal reads ratio of libraries prepared with gDNA samples (Illumina platform) with different library preparation schemes;B. Abnormal reads ratio of libraries prepared with different grades of FFPE DNA samples by using enzymatic fragmentation library preparation kit (MGI platform). Note: Artifact rate: the ratio of abnormal sequence introduced by enzymatic digestion by-products.
Excellent Capture Performance
Figure 6 The capture performance of libraries prepared by the enzyme-based kit and the sonication-based kit.The libraries were prepared from 50 ng of FFPE DNA samples with different grades, using either NadPrep Sonication strategy or NadPrep Enfrag strategy, and the multi-hybridization reaction was initiated with 500 ng of each library with the same library preparation scheme by using NanOnco Plus Panel v2.0 (2.6 M) coupled with NadPrep Hybrid Capture Reagents and NadPrep NanoBlockers (for Illumina®), and the sequencing data is analyzed by selecting 2 M reads pair randomly. The performance of capture data of different library preparation schemes was basically consistent inA.Mappability and On-target rate, but the performance in B. Fold 80 penalty,C. Duplication rate and D. Mate on differ chr was superior to that of competitive products.
Library Prep Module
Package | Color of Tube Cap | Component | Volume 1002601(24 rxn) |
Volume 1002602 (96 rxn) |
Box1 | FERA Buffer | 150 µL | 575 µL | |
FERA Enzyme | 150 µL | 575 µL | ||
Enhancer Buffer | 250 µL | 950 µL | ||
Ligation Buffer | 750 µL | 2×1500 µL | ||
DNA Ligase | 65 µL | 250 µL | ||
2X HiFi PCR Master Mix | 720 µL | 2×1600 µL | ||
/ | Nuclease Free Water | 2.5 mL | 8 mL | |
TE Solution | 1 mL | 4 mL | ||
Box2 | / | NadPrep SP Beads | 4 mL | 15 mL |
Single index(SI) adapter module
Color of Tube Cap | Component | Volume 1003611 (24 rxn) |
Volume 1003621 (96 rxn) |
Volume 1003622(96 rxn) |
NadPrep M-Index (SI) Primer Mix # | 12×15 µL | 24×25 µL | 24×25 µL | |
NadPrep M-Adapter | 60 µL | 230 µL | 230 µL |
Color of Tube Cap | Component | Volume
1003641 (96 rxn) |
NadPrep M-Index (SI) Primer Mix # | 96×8 µL (Plate) | |
NadPrep M-Adapter (SI) | 260 µL (Tube) | |
NadPrep M-Amplification Primer Mix (for MGI, SI) | 75 µL (Tube) |
Unique Dual Index (MDI) Adapters
Color of
Tube Cap |
Component | Volume 1003711 (24 rxn) |
Volume 1003721 (96 rxn) |
Volume 1003722(96 rxn) |
Volume 1003725 (96 rxn) |
Volume 1003726 (96 rxn) |
NadPrep M-Index (MDI) Primer Mix # | 12×15 µL | 24×25 µL | 24×25 µL | 24×25 µL | 24×25 µL | |
NadPrep M-Adapter | 60 µL | 230 µL | 230 µL | 230 µL | 230 µL |
Color of Tube Cap | Component | Volume 1003751* (96 rxn) |
Volume 1003752* (96 rxn) |
Volume 1003753* (96 rxn) |
Volume 1003754* (96 rxn) |
NadPrep M-Index (MDI) Primer Mix # | 96×8 µL (Plate) | 96×8 µL (Plate) | 96×8 µL (Plate) | 96×8 µL (Plate) | |
NadPrep M-Adapter (DI) | 260 µL (Tube) | 260 µL (Tube) | 260 µL (Tube) | 260 µL (Tube) | |
NadPrep M-Amplification Primer Mix (for MGI, DI) | 75 µL (Tube) | 75 µL (Tube) | 75 µL (Tube) | 75 µL (Tube) |
Color of Tube Cap | Component | Volume 1003761 (1152 rxn) |
Volume 1003762 (1152 rxn) |
Volume 1003763 (1152 rxn) |
Volume 1003764 (1152 rxn) |
NadPrep M-Index (MDI) Primer Mix # | 96×72 µL (Tube) | 96×72 µL (Tube) | 96×72 µL (Tube) | 96×72 µL (Tube) | |
NadPrep M-Adapter (DI) | 2×1350 µL (Tube) | 2×1350 µL (Tube) | 2×1350 µL (Tube) | 2×1350 µL (Tube) | |
NadPrep M-Amplification Primer Mix (for MGI, DI) | 900 µL (Tube) | 900 µL (Tube) | 900 µL (Tube) | 900 µL (Tube) |
NadPrep UDI Adapter Module
Color of Tube Cap | Component | Volume 1003211 (24 rxn) |
Volume 1003222 (24 rxn) |
Volume 1003211 (96 rxn) |
Volume 1003212 (96 rxn) |
Volume 1003213 (192 rxn) |
Volume 1003224 (480 rxn) |
NadPrep UDI Adapter # | 12×10 µL | 12×10 µL | 12×22 µL | 12×22 µL | 24×22 µL | 96×3.5 µL (Plate)×5 |
Universal Stubby Adapter (UDI) Module
Color of Tube Cap | Component | Volume 1003240 (24 rxn) |
Volume 1003241 (96 rxn) |
Volume 1003242 (96 rxn) |
NadPrep Universal UDI-index Primer Mix # | 12×15 µL | 24×25 µL | 24×25 µL | |
NadPrep Universal Stubby Adapter | 60 µL | 230 µL | 230 µL | |
NadPrep Amplification Primer Mix II | 20 µL | 75 µL | 75 µL |
Color of Tube Cap | Component | Volume 1003251 (96 rxn) |
Volume 1003252 (96 rxn) |
Volume 1003253 (96 rxn) |
Volume 1003254 (96 rxn) |
Volume 1003255 (96 rxn) |
Volume 1003256 (96 rxn) |
Volume 1003257 (96 rxn) |
Volume 1003258 (96 rxn) |
NadPrep Universal UDI-index Primer Mix # | 96×8 µL (Plate) | 96×8 µL (Plate) | 96×8 µL (Plate) | 96×8 µL (Plate) | 96×8 µL (Plate) | 96×8 µL (Plate) | 96×8 µL (Plate) | 96×8 µL (Plate) | |
NadPrep Universal Stubby Adapter | 260 µL (Tube) | 260 µL (Tube) | 260 µL (Tube) | 260 µL (Tube) | 260 µL (Tube) | 260 µL (Tube) | 260 µL (Tube) | 260 µL (Tube) | |
NadPrep Amplification Primer Mix II | 75 µL (Tube) | 75 µL (Tube) | 75 µL (Tube) | 75 µL (Tube) | 75 µL (Tube) | 75 µL (Tube) | 75 µL (Tube) | 75 µL (Tube) |
Color of Tube Cap | Component | Volume
1003261 (1152 rxn) |
Volume
1003262 (1152 rxn) |
Volume
1003263 (1152 rxn) |
Volume
1003264 (1152 rxn) |
NadPrep Universal UDI-index Primer Mix # | 96×72 µL (Tube) | 96×72 µL (Tube) | 96×72 µL (Tube) | 96×72 µL (Tube) | |
NadPrep Universal Stubby Adapter | 2×1350 µL (Tube) | 2×1350 µL (Tube) | 2×1350 µL (Tube) | 2×1350 µL (Tube) | |
NadPrep Amplification Primer Mix II | 900 µL (Tube) | 900 µL (Tube) | 900 µL (Tube) | 900 µL (Tube) |
Type | Product | Scale | Catalog |
Library Module | NadPrep EZ DNA Library Preparation Module v2, 24 rxn | 24 rxn | 1002601 |
NadPrep EZ DNA Library Preparation Module v2, 96 rxn | 96 rxn | 1002602 | |
Universal Adapter (SI) Module | NadPrep Universal Adapter (SI) Module Set A1 (for MGI), 24 rxn | 24 rxn | 1003611 |
NadPrep Universal Adapter (SI) Module Set B1 (for MGI), 96 rxn | 96 rxn | 1003621 | |
NadPrep Universal Adapter (SI) Module Set B2 (for MGI), 96 rxn | 96 rxn | 1003622 | |
NadPrep Universal Adapter (SI) Module Set E1 (for MGI), 96 rxn | 96 rxn | 1003641 | |
Universal Adapter (MDI) Module | NadPrep Universal Adapter (MDI) Module Set A1 (for MGI), 24 rxn | MDI 1-12 | 1003711 |
NadPrep Universal Adapter (MDI) Module Set B1 (for MGI), 96 rxn | MDI 1-24 | 1003721 | |
NadPrep Universal Adapter (MDI) Module Set B2 (for MGI), 96 rxn | MDI 25-48 | 1003722 | |
NadPrep Universal Adapter (MDI) Module Set B3 (for MGI), 96 rxn | MDI 49-72 | 1003725 | |
NadPrep Universal Adapter (MDI) Module Set B4 (for MGI), 96 rxn | MDI 73-96 | 1003726 | |
NadPrep Universal Adapter (MDI) Module Set E1 (for MGI), 96 rxn | MDI 1-96 | 1003751 | |
NadPrep Universal Adapter (MDI) Module Set E2 (for MGI), 96 rxn | MDI 97-192 | 1003752 | |
NadPrep Universal Adapter (MDI) Module Set E3 (for MGI), 96 rxn | MDI 193-288 | 1003753 | |
NadPrep Universal Adapter (MDI) Module Set E4 (for MGI), 96 rxn | MDI 289-384 | 1003754 | |
NadPrep Universal Adapter (MDI) Module Set F1 (for MGI), 1152 rxn | MDI 1-96 | 1003761 | |
NadPrep Universal Adapter (MDI) Module Set F2 (for MGI), 1152 rxn | MDI 97-192 | 1003762 | |
NadPrep Universal Adapter (MDI) Module Set F3 (for MGI), 1152 rxn | MDI 193-288 | 1003763 | |
NadPrep Universal Adapter (MDI) Module Set F4 (for MGI), 1152 rxn | MDI 289-384 | 1003764 | |
Universal Stubby Adapter (UDI) Module | NadPrep Universal Stubby Adapter (UDI) Module Set A1, 24 rxn | Index 1-12 | 1003240 |
NadPrep Universal Stubby Adapter (UDI) Module Set B1, 96 rxn | Index 1-24 | 1003241 | |
NadPrep Universal Stubby Adapter (UDI) Module Set B2, 96 rxn | Index 25-48 | 1003242 | |
NadPrep Universal Stubby Adapter (UDI) Module Set E1, 96 rxn | Index 1-96 | 1003251 | |
NadPrep Universal Stubby Adapter (UDI) Module Set E2, 96 rxn | Index 97-192 | 1003252 | |
NadPrep Universal Stubby Adapter (UDI) Module Set E3, 96 rxn | Index 193-288 | 1003253 | |
NadPrep Universal Stubby Adapter (UDI) Module Set E4, 96 rxn | Index 289-384 | 1003254 | |
NadPrep Universal Stubby Adapter (UDI) Module Set E5, 96 rxn | Index 385-480 | 1003255 | |
NadPrep Universal Stubby Adapter (UDI) Module Set E6, 96 rxn | Index 481-576 | 1003256 | |
NadPrep Universal Stubby Adapter (UDI) Module Set E7, 96 rxn | Index 577-672 | 1003257 | |
NadPrep Universal Stubby Adapter (UDI) Module Set E8, 96 rxn | Index 673-768 | 1003258 | |
NadPrep Universal Stubby Adapter (UDI) Module Set F1, 1152 rxn | Index 1-96 | 1003261 | |
NadPrep Universal Stubby Adapter (UDI) Module Set F2, 1152 rxn | Index 97-192 | 1003262 | |
NadPrep Universal Stubby Adapter (UDI) Module Set F3, 1152 rxn | Index 193-288 | 1003263 | |
NadPrep Universal Stubby Adapter (UDI) Module Set F4, 1152 rxn | Index 289-384 | 1003264 | |
UDI Adapter Module | NadPrep UDI Adapter Kit Set A (for Illumina®), 96 rxn | Index 1-12 | 1003211 |
NadPrep UDI Adapter Kit Set B (for Illumina®), 96 rxn | Index 13-24 | 1003212 | |
NadPrep UDI Adapter Kit Set A&B (for Illumina®), 192 rxn | Index 1-24 | 1003213 | |
NadPrep UDI Adapter Kit Set C1 (for Illumina®), 24 rxn | Index 1-12 | 1003221 | |
NadPrep UDI Adapter Kit Set D1 (for Illumina®), 480 rxn | Index 1-96 | 1003224 |
[Product Catalog] – Product Catalog
[Technical Note] – EN_Protocol_NadPrep_EZ_DNA_Library_Preparation_Kit_for_Illumina_V2.3
[Technical Note] – EN_Protocol_NadPrep_EZ_DNA_Library_Preparation_Kit_for_MGI_V2.2
[Technical Note] – NadPrep EZ DNA Library Preparation Kit (for MGI) Protocol
[Poster] – NadPrep_EZ_DNA_Library_Preparation_Module_v2
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