Clean Quick Plant

DNA Integrity
To check the integrity of extracted DNA, Clean Quick Plant DNA extraction was performed on 300 µL supernatant of grinded and lysed sunflower and wheat plant seeds.The size distribution of the extracted DNA visualized on an agarose gel indicates high DNA integrity (Figure 1) .

Figure 1
Agarose gel electrophoresis analysis of genomic DNA extracted from different crop seeds (10 µL per sample).
Lanes 1-3 contain DNA from sunflower seeds and lanes 4-6 contain DNA from wheat seeds. Lane 7 contains a 1kb DNA ladder.

DNA Purity
In addition to the agarose gel analysis, OD measurements were performed to show the purity of the DNA extracted from sunflower and wheat seeds. The 260/230 ratio greater than 2.0 and 260/280 ratio greater than 1.8 indicate that the extracted DNA is free of protein, polyphenols or other contaminants, making it suitable for all downstream applications indicated (Table 1).

Table 1
OD measurements of eluted DNA comparing DNA yield and purity from sunflower and wheat seeds.

In another experiment, DNA was extracted from tomato and cucumber leaves. After grinding, lysing and centrifuging, 100 μL of supernatant was used for extraction with Clean Quick Plant. Similar to the OD values of the DNA extracted from plant seeds, the 260/230 ratio greater than 2.0 and 260/280 ratio greater than 1.8 indicating that the DNA extracted from leaves is free of contaminants and suitable for all downstream applications indicated (Table 2).

Table 2
OD measurements of eluted DNA comparing DNA yield and purity from tomato and cucumber leaves.