Clean Cell Free DNA Kit (CE-IVD)

Cell-free DNA (cfDNA) has gained significant attention as a diagnostic and research tool in medicine. In recent years, cfDNA has revolutionized prenatal diagnostics by enabling non-invasive prenatal testing (NIPT). Moreover, it is quickly becoming the new diagnostic standard for oncology, transplant medicine, and cardiovascular diseases. Our CE-IVD marked Clean Cell Free DNA Kit assists laboratories with the crucial first step of the diagnostic process: extracting cfDNA from human plasma.

Our kit enables the extraction of cfDNA fragments from 1 mL of human plasma through a straightforward, three-step procedure that is easy to automate. The unique lysis buffer effectively releases DNA from proteins and vesicles, while the optimized buffer combination enriches DNA fragments ranging from 120 to 400 base pairs. Coupled with the CE-IVD marking, the Clean Cell Free DNA Kit is the ideal solution for achieving reliable and reproducible qPCR results in molecular diagnostics.

Workflow 

Initially, the uniquely formulated lysis buffer is added to the plasma sample to release the cfDNA from proteins and vesicles, while DNases and RNases are inactivated. After binding of the cfDNA to the added magnetic beads, the cfDNA-magnetic bead complex can be separated from the lysate by using a magnetic separation device. A series of quick wash steps remove trace contaminants and the purified cfDNA can be eluted from the magnetic beads using the Elution Buffer.