Air-Dryable™ Direct DNA LAMP Blood mix is a ready-to-use, 4x concentrated, fully optimized mix for isothermal amplification methods such as loop-mediated isothermal amplification (LAMP) on blood. It is designed to dramatically speed up assay development time and reduce time to results. The glycerol-free mix contain optimized excipients compatible with air drying, making it ideal for POC blood testing. Can also be used wet and/or with purified nucleic acid
Air-Dryable™ Direct DNA LAMP Blood mix is a ready-to-use, 4x concentrated, fully optimized mix for isothermal amplification methods such as loop-mediated isothermal amplification (LAMP) on blood. It is designed to dramatically speed up assay development time and reduce time to results. The glycerol-free mix contain optimized excipients compatible with air drying, making it ideal for POC blood testing. Can also be used wet and/or with purified nucleic acid
Higher Specificity and Quicker Time to Detect Viral Targets
(A) Air-Dryable™ Direct DNA LAMP Blood (MDX126, red) was air-dried with primers for Epstein-Barr virus (EBV), Hepatitis B Virus (HBV), Herpes Simplex Virus 2 (HSV2), Trichomonas vaginalis and Mycoplasma pneumoniae. The performance of the mix was compared against NEB WarmStart® LAMP kit (orange) and Thermo SuperScript™ IV RT-LAMP Master Mix (black) using the same primers and DNA. Reactions were incubated at 65°C for 60 minutes. Error bars represent the standard deviation across three technical replicates. The Air-Dryable™ Direct LAMP Blood mix delivered faster target amplification (TTR) with higher specificity, as demonstrated by the longest delay in no-template control (NTC) amplification across range of pathogenic DNA targets.
Blood is one of the most common specimens used for laboratory diagnostic testing and it is useful for diagnosing diseases such as bacterial and viral infections, cancer, cardiovascular disease, and metabolic disorders such as diabetes. However, whole blood specimens, serum, and plasma contain a number of inherent PCR inhibitors including immunoglobulin G, hemoglobin, lactoferrin, and leukocyte DNA. In addition, PCR inhibitors can be found in the anticoagulants used to stabilize blood samples (e.g. EDTA, citrate, or heparin). Isothermal amplification such as LAMP is very tolerant to inhibitors and so is ideal for point of care (POC) testing, the simple workflow allows fast, sensitive and reliable results in clinical diagnosis.
The Air-Dryable Direct DNA LAMP Blood mix combines even greater tolerance with fast, robust isothermal amplification to enable highly sensitive direct detection using crudely processed whole blood, plasma, or serum samples. The mix are uniquely designed to overcome much higher concentrations of inhibitors, and so do not require a DNA purification step to concentrate and purify a patient sample. Very fast reaction kinetics result in faster time to results and superior amplification sensitivity and specificity. In addition, the 4x concentrated master mixes are formulated with excipients which are optimized for air drying to create ambient-temperature stable assays, making them ideal for POC blood testing.
Air-Dryable Direct DNA LAMP Blood, 4x, MDX126
Application: LAMP Specimen Type: Blood, Plasma, Serum, DNA Concentration: 4x High Concentration: Yes Wet: Liquid
Glycerol-Free: Yes Lyo-Ready: No Air-Dryable: Yes Dryable: Air-Dryable Sustainability: Air-Dryable
Inhibitor tolerance to 10% whole blood, 10% plasma, compatible with all anticoagulants. Perfect for multiplexing and fast cycling conditions. Used in the detection of blood pathogens and liquid biopsy applications.
Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots
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