The Lyo-Ready LAMP Mixes are 4x master mix formulated with excipients optimized for freeze-drying to create ambient-temperature stable assays. Given cold chain management is a major limiting factor in resource-limited settings, ambient-temperature stable point-of-care tests (POCT) offer several advantages, including no refrigeration, reduced shipping, and handling costs, and extended shelf-life. By using Meridian’s Lyo-Ready LAMP Mixes, the process of developing a sensitive and high-performing assay is made significantly easier, with the added flexibility of using the mix in a wet or dried-down format.
Meridian’s Lyo-Ready LAMP/RT-LAMP Mixes are ready-to-use, 4x concentrated, fully optimized mixes for isothermal amplification methods such as Loop mediated isothermal amplification (LAMP), designed to dramatically speed up assay development time and reduce time to results. Loop mediated isothermal amplification is a highly sensitive nucleic acid amplification method that has gained popularity in recent years due to its advantages over qPCR, such as quicker time to results, simple assay workflow, and point-of-care usability. However, the initial assay design and development can be more complex with 4 to 6 primers which are used in combination to generate a robust, fast, and sensitive result. Through extensive R&D research, Meridian has discovered a blend of bst DNA polymerase, reverse transcriptase, dNTPs, Mg2+, excipients, and stabilizers that are used with customer-selected primers and probes for creating any custom DNA or RNA LAMP test. Experimental data on both RNA and DNA templates demonstrate that the mixes are up to 2x faster than other suppliers, generating results in half the time. In addition, the mixes incorporate Meridian’s Lyo-Ready technology and can be used in a liquid format or lyophilized to create an ambient-temperature stable assay (please see the product guide and FAQs for recommendations on lyophilization parameters). By using a ready-to-use master mix such as Lyo-Ready LAMP Mix or Lyo-Ready RT-LAMP Mix, LAMP test assay development is faster, simpler, and more efficient, speeding up your product development time and allowing you to commercialize your new products faster. Can also be used wet
Glycerol-free mix for isothermal applications such as RT-LAMP, containing Bst DNA polymerase, reverse transcriptase, reaction buffer, dNTPs and lyo-compatible excipients, ideal for developing ambient temperature, LAMP assays.
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