Lyo-Ready Direct DNA qPCR and RNA/DNA qPCR Urine mixes are glycerol-free mixes that contain optimized excipients compatible with lyophilization. The mixes have been designed for the development of quantitative PCR urine test assays for direct detection of DNA and RNA from crudely processed human urine specimens. Can also be used wet and/or with purified nucleic acid
Urine is an ideal clinical specimen because it is excreted in large quantities, is non-invasive, and it can be self-sampled. Urine specimens are used in the diagnosis and management of infectious diseases, bladder cancer and urinary tract infections (UTIs). However, urine contains substances such as urea and nucleases that can damage DNA or inhibit the PCR reaction.
Meridian’s new Lyo-Ready Direct Urine molecular mixes are unique in that they enable highly sensitive, very fast detection of target DNA or RNA directly from high concentrations of urine. With the CDC reporting a 30% increase in reportable sexually transmitted disease (STDs) between 2015 and 2019, these direct urine molecular mixes are ideal, as they allow less invasive sample collection (than a cervical or urethra swab) and encourage routine screening. They have been formulated specifically to overcome the inhibitors found in urine – no further optimization is required aside from the addition of primers and probes. Furthermore, these mixes can be used in a wet format or dried down by lyophilization to create ambient-temperature stable assays.
Inhibitor tolerance to 20% urine. Perfect for multiplexing and fast cycling conditions. Used in the detection of urinary track infections, STD, tropical diseases, cancer screening, bladder and prostate cancer.
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