Lyo-Ready Direct DNA qPCR and RNA/DNA qPCR Saliva mixes are glycerol-free mixes that contain optimized excipients compatible with freezing and lyophilization and they designed for the direct quantitative PCR saliva testing of DNA and RNA. Can also be used wet and/or with purified nucleic acid.
Over the last decade, saliva has been extensively studied as a diagnostic specimen due to its non-invasive and simple sample collection/logistics. Like serum, saliva contains hormones, antibodies, growth factors, enzymes, microbes that can be used as biomarkers for disease detection. However, there have been challenges in adopting a saliva qPCR test for molecular diagnostics due to the low concentration of analytes and high concentration of PCR inhibitors in saliva, which typically requires cumbersome sample preparation steps to homogenize the specimen and purify the DNA/RNA.
Lyo-Ready Direct DNA qPCR Saliva is a 4x master mix for the development of DNA saliva testing, enabling highly sensitive detection of target DNA from crudely processed saliva or sputum samples. Lyo-Ready Direct RNA/DNA qPCR Saliva is 4x master mix and can be used for the development of both RNA and DNA saliva testing from crudely processed saliva or sputum samples. These mixes have been formulated to overcome the inhibitors specifically found in saliva samples – no further optimization is required aside from the addition of primers and probes. Furthermore, these mixes can be used in a wet format or lyophilized to create ambient-temperature stable saliva tests or sputum tests.
Inhibitor tolerance to 60% crude saliva, sputum or mucin samples and 50% UTM. Perfect for multiplexing and fast cycling conditions. Used in the detection of a broad range of diseases, including cancer, respiratory viruses and bacterial infections.
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