Lyo-Ready Direct DNA and RNA/DNA LAMP Saliva mixes are glycerol-free mixes that contain optimized excipients compatible with lyophilization and they are designed for direct loop mediated isothermal amplification for saliva testing of DNA and RNA. Can also be used wet and/or with purified nucleic acid
Saliva is ideal for use as a diagnostic specimen as it contains hormones, antibodies, growth factors, enzymes and microbes that can be used as biomarkers for disease detection. Saliva is easy-to-collect, making it perfect for point-of-care saliva testing. However, due to the low concentration of analytes and high concentration of inhibitors, the use of molecular diagnostic saliva testing has been limited. Isothermal amplification such as LAMP is more tolerant to inhibitors and the simple workflow allows fast, sensitive, reliable results in clinical diagnosis.
Lyo-Ready DNA Direct LAMP Saliva is a 4x RT-qPCR master mix for the development of DNA saliva and sputum tests, enabling highly sensitive detection of target DNA from crudely processed samples. Lyo-Ready RNA/DNA Direct LAMP Saliva is a 4x RT-qPCR master mix and can be used for the development of both RNA and DNA saliva and sputum tests from crudely processed samples. These mixes have been formulated specifically to overcome the inhibitors found in saliva samples – no further optimization is required aside from the addition of primers and probes. Furthermore, these mixes can be used in a wet format or dried down by lyophilization to create ambient-temperature stable saliva tests or sputum tests.
Inhibitor tolerance to 60% crude saliva, sputum or mucin samples and 50% UTM. Perfect for multiplexing and fast cycling conditions. Used in the detection of a broad range of diseases, including cancer, respiratory viruses and bacterial infections.
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