Lyo-Ready 1-Step RT-qPCR Virus Mix is a glycerol-free mix, ideal for developing ambient temperature, lyophilized, multiplex qPCR and RT-qPCR molecular diagnostic tests, particularly for RNA viruses, and is suited for high-throughput, automated platforms or microfluidic qPCR platforms requiring millisecond rehydration times. Can also be used wet
Molecular diagnostic tests are progressively moving towards lyophilized formats. There are several advantages for this, including ambient temperature shipping and storage, extended shelf-life, reduced operating steps and potential errors and increased flexibility in sample volume. In order to be compatible with drying, however, enzyme preparations must be glycerol-free and include specialized excipients that preserve the mixture as it is exposed to various conditions including freezing, vacuum, and dehydration.
Lyo-Ready 1-Step RT-qPCR Virus Mix is a 2x RT-qPCR master mix and can be used for the development of both RNA and DNA based molecular diagnostic tests, it contains a separate lyo-compatible MMLV-RT and enzyme dilution buffer. The Lyo-Ready 1-Step RT-qPCR Virus Mix reverse transcriptase is also thermostable and has been optimized for amplification of DNA or RNA viruses with a high secondary structure (reverse transcriptase remains active at 55-60 °C).
Taq polymerase, RNase inhibitor, reaction buffer, dNTP, MgCl2 and lyo-excipients and separate reverse transcriptase, optimized for amplification of RNA with high secondary structure.
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