Low LOD 1-Step RT-qPCR Mix is fast, highly reproducible real-time PCR master mix that has been designed with a highly optimized buffer chemistry and hot-start DNA polymerase, to deliver excellent results and very low background, allowing greater sensitivity.
Low LOD 1-Step RT-qPCR master mix contains Taq DNA polymerase, reaction buffer, dNTP, MgCl2, stabilizers, and PCR enhancers, and a separate RNase tolerant reverse transcriptase mix. However, the dTTP in the dNTPs has been substituted for dUTP, so that Uracil DNA Glycosylase (UDGase) (MDX054) can be added to remove background PCR product contamination. This makes Low LOD 1-Step RT-qPCR ideal for developing highly sensitive RT-qPCR diagnostic tests, such as for detecting RNA and DNA viruses at very low levels in blood banks or transplant viral testing.
RT-qPCR mix a dNTP/dUTP mix, designed for stringent amplification of highly structured and very low copy-number RNA and DNA targets.
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