T7 RNA Polymerase is a DNA-dependant RNA Polymerase originating from the T7 bacteriophage and is the industry standard enzyme for in vitro transcription (IVT). Not only does T7 RNA Polymerase provide for mRNA vaccine production, but it can also be used for other RNA-based therapeutic and research applications, including miRNA and siRNA synthesis for RNA interference drugs, synthesis of sgRNA for the CRISPR/Cas9 system and in isothermal diagnostic methods, such as nucleic acid sequence-based amplification (NASBA).
Glycerol-Free T7 RNA Polymerase (HC) is a high concentration (1,000 U/µL) DNA-dependent RNA Polymerase with very high specificity for the T7 phage double-stranded promoter sequence. The enzyme catalyzes the 5’→3’ synthesis of RNA on either single-stranded DNA or double-stranded DNA downstream from the promoter. Glycerol-Free T7 RNA Polymerase (HC) has been optimized to deliver excellent, stable performance without glycerol, allowing you to develop in vitro transcription workflows with a high-quality enzyme that delivers reliable results with the added capability for lyophilization. This gives you the flexibility to confidently store reaction mixtures at ambient temperature or produce diagnostic assays that rely on miniaturized reaction components.
Glycerol-free, high concentration DNA-dependant RNA Polymerase that requires a T7 promoter sequence, it an ideal enzyme for in-vitro synthesis of labeled or unlabeled RNA for research, diagnostic or clinical applications. Ideal for lyophilization.
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