Meridian Bioscience (MDx)

Glycerol-Free T4 DNA Ligase (HC)

Glycerol-Free T4 DNA Ligase (HC) is a glycerol-free, high concentration enzyme (50U/µL) that catalyzes the formation of a phosphodiester bond between adjacent 5ʹ phosphate and 3′ hydroxyl groups in duplex DNA or RNA. The enzyme efficiently joins blunt and cohesive ends and repairs single stranded nicks in duplex DNA, RNA or DNA/RNA hybrids.

Features:

  • Efficiently joins blunt and cohesive ends.
  • Repairs single-stranded nicks in duplex DNA, RNA and DNA/RNA hybrids.
  • Joins double-stranded oligonucleotide linkers or adaptors to DNA, for use in NGS library construction and cloning.
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