Fast 1-Step RT-qPCR master mix contains Taq polymerase, reaction buffer, dNTP, MgCl2, stabilizers, PCR enhancers, and separate reverse transcriptase and RNase inhibitor. It is ideal for developing multiplex one-step qPCR diagnostic tests and is suited for high-throughput, automated platforms.
A 10-fold serial dilution of human cDNA amplified with four different probes; both in singleplex reactions (blue line) and quadruplex reaction (the red line displayed is for the same primers as for the singleplex). Five replicates were run (10 min 45°C followed by 2 min 95°C and 45 cycles 95°C 5s, 20°C 10s) and the results illustrate the high sensitivity, excellent reproducibility and Ct values for both the singleplex and multiplex reactions with no reduction of efficiency that is often associated with multiplexing.
A combination of the latest advances in buffer chemistry and PCR enhancers, together with a hot-start polymerase, dNTPs and MgCl2 and separate reverse transcriptas, ideal for fast, accurate, highly reproducible RT-qPCR.
Please fill out the following form about your inquiry.
