Air-Dryable™ Direct DNA qPCR and RNA/DNA qPCR Saliva master mixes are glycerol-free and contain optimized excipients compatible with oven drying and are designed for the direct qPCR assays. Can also be used wet and/or with purified nucleic acid
Over the last decade, saliva has been extensively studied as a diagnostic specimen due to its non-invasive and simple sample collection, and like serum, saliva contains hormones, antibodies, growth factors, enzymes, microbes that can be used as biomarkers for disease detection. However, there have been challenges in adopting saliva for molecular diagnostics due to the low concentration of analytes and high concentration of PCR inhibitors in saliva. Up until now cumbersome sample preparation steps have been required to homogenize the specimen and adequately purify the DNA/RNA.
Air-Dryable™ DNA qPCR Saliva is a 4x qPCR master mix for the development of DNA saliva tests, enabling highly sensitive detection of target DNA from crudely processed saliva or sputum samples. Air-Dryable™ RNA/DNA qPCR Saliva is 4x RT-qPCR master mix designed for the development of both RNA and DNA saliva tests from crudely processed saliva or sputum samples. These mixes have been formulated specifically to overcome the inhibitors found in saliva samples – no further optimization is required aside from the addition of primers and probes. Furthermore, these mixes can be used in a wet format or dried down by oven to create ambient-temperature stable tests.
Inhibitor tolerance to 60% crude saliva, sputum or mucin samples and 50% UTM. Perfect for multiplexing and fast cycling conditions. Used in the detection of a broad range of diseases, including cancer, respiratory viruses and bacterial infections.
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