Meridian’s 55C MMLV-RT is a highly robust, thermostable reverse transcriptase, ideal for cDNA synthesis from RNA with a high secondary structure such as viral RNA genomes.
Traditional Moloney Murine Leukemia Virus Reverse Transcriptase (MMLV-RT) is not thermostable and can only maintain its enzymatic activity at relatively low temperatures (up to 50°C). However, for cDNA synthesis, a higher reaction temperature is sometimes desirable as it reduces RNA secondary structures which can inhibit reverse transcription and it minimizes nonspecific primer binding. 55C MMLV-RT is a highly thermostable reverse transcriptase with reduced RNase H activity, that allows very efficient first-strand cDNA synthesis at temperatures up to 60°C. This improves the cDNA yield from difficult RNA targets, such as viral RNA genomes, that require higher temperature to denature strong RNA secondary structure.
Highly robust, thermostable reverse transcriptase, ideal for cDNA synthesis from RNA with a high secondary structure such as viral RNA genomes.
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