Tetro Reverse Transcriptase

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Tetro Reverse Transcriptase

Tetro™ Reverse Transcriptase is a highly sensitive, high stability MMLV reverse transcriptase. Tetro Reverse Transcriptase is optimized for reverse transcription reactions using a wide range of total RNA amounts, such that long and low abundance cDNAs can be detected by amplification after cDNA synthesis.

Tetro Reverse Transcriptase is suitable for first-strand cDNA synthesis, cDNA library construction, and the production of templates for RT-PCR analysis of gene expression.

Product Highlights

  • Highly sensitive – for high-quality, full length cDNA from as little as 10 pg of total RNA
  • Ultra-stable – for long genes and rare transcripts
  • High yield – produces high quality cDNA ideal for PCR
  • Broad dynamic range – 10 pg to 5 μg of RNA

Product Description

Tetro™ Reverse Transcriptase is a Moloney Murine Leukaemia Virus (MMLV) Reverse Transcriptase, which exhibits high stability, with no loss of activity following 1 week at room temperature. Tetro Reverse Transcriptase is highly sensitive even when the amount of template is a limiting factor , with highly efficient and sensitive transcription, from as little as 10 pg, up to 5 μg of RNA.

Many RNA transcripts form stable secondary structures at lower temperatures, making them less suitable as templates for RT-PCR at those temperatures.

Tetro Reverse Transcriptase is suitable for first-strand cDNA synthesis, with total RNA, mRNA and in vitro transcribed RNA and shows excellent performance with gene-specific primers, Oligo (dT) as well as random hexamers, making it perfect for cDNA library construction and the production of templates for RT-PCR analysis of gene expression.

 

SKU:  Size: Webshop:
BIO-65050 10 000 Units Order online

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