Formulated for highly reproducible first-strand cDNA synthesis and subsequent PCR in a single tube.
• Sensitive – incorporates a blend of high-affinity RT and novel MyTaq HS DNA Polymerase, enabling amplification of low-copy number targets from ≥3 pg total RNA
• Efficient – novel one-step buffer system maximizes the efficiency of both the reverse transcription and PCR steps, delivering improved yield of any target
• Robust – RT tolerates the higher reaction temperatures required to overcome secondary structure, giving reliable detection of even challenging and GC-rich targets
• Specific – MyTaq HS DNA Polymerase is an antibody-mediated hot-start enzyme that remains completely inactive during PCR set-up to prevent non-specific amplification
• Flexible – utilizes gene-specific primers for full-length reverse transcription and subsequent PCR amplification of any RNA target
• Convenient – an all-in-one-tube mastermix that improves the speed, convenience and accuracy of RT-PCR
MyTaq™ One-Step RT-PCR Kit incorporates the latest advances in buffer chemistry, including Meridian ultra-pure dNTPs, together with a proprietary reverse transcriptase and MyTaq HS, a new generation of antibody-mediated hot-start DNA polymerase. This ensures that MyTaq One-Step RT-PCR Kit enables fast, highly-specific and ultra-sensitive amplification of RNA targets for use in a broad range of downstream applications.
MyTaq One-Step Kit is ideal for determining the presence or absence of RNA templates and quantifying expression through qualitative or semi-quantitative analysis of RNA transcription levels. The one-step format uses gene specific primers to maximize amplification of these targets, whilst eliminating non-specific amplification, ensuring highly efficient and sensitive transcription from as little as 3 pg total RNA.
MyTaq One-Step RT-PCR Kit is perfect for the synthesis of double-stranded cDNA products for subsequent gene expression analysis over a broad temperature range, the use of higher temperatures allows reverse transcription through RNA secondary structure, including difficult and GC-rich sequences. Enhanced amplification of these targets ensures high cDNA yields from all RNA, including total RNA, mRNA, in vitro transcribed RNA, snRNA and viral RNA.
| SKU: | Size: | Webshop: |
| BIO-65048 | 25 x 50µl Reactions | Order online |
| BIO-65049 | 100 x 50µl Reactions | Order online |
All components should be stored at -20°C upon receipt for optimum stability. Repeated freeze/thaw cycles should be avoided.
When stored under the recommended conditions and handled correctly, full activity of the reagents is retained until the expiry date indicated on the outer box label.
Brochure
Please fill out the following form about your inquiry.
We are experts in Life Science Automation that understand our customers and suppliers. Trust and quality are the basis for the partnerships we form.
