The Singulator™ 100 System
Solid Tissue Dissociation.
The bench-top Singulator System and its single-use cartridges enable reproducible, rapid and hands-off tissue dissociations into single-cell or nuclei suspensions. Its ability to perform cold dissociation minimizes the expression of stress-related genes in cells and helps preserve RNA quality in nuclei. Researchers can now easily obtain suspensions of nuclei or high-viability cells for a wide range of single-cell analyses.
Minimal Variability. Multiple Applications.
Ideal for genomics, cell biology and other ‘omics applications, including scRNA-Seq, snRNA-Seq, ATAC-Seq, CITE-Seq, FACS, and immuno-oncology. S2 Genomics provides a selection of pre-set protocols and pre-formulated reagents for cell isolations for an expanding set of mouse, rat, and human tissues, including tumors. See the wide range of tissues and organisms demonstrated on the Singulator 100 System here.
Process fresh, frozen and OCT tissues.
|TISSUE TYPE||PROCESS TIME||YIELD*||VIABILITY*|
|Cells||Fresh||20-60 minutes||10,000 to >500,000/mg||70-95%|
|Nuclei||Fresh, Frozen, OCT||5-1/2 minutes||10,000 to >600,000/mg||N/A|
*Varies depending on tissue types
Nuclei In 7-12 Minutes
Reproducibly generate high-quality suspensions of nuclei from a wide range of tissues.
Merged DAPI-stained and bright-field images of small intestine, brain and heart tissue nuclei; DAPI stained liver nuclei. Courtesy of Dr. Minoda, Laboratory for Cellular Epigenomics, RIKEN Yokohama, Japan.
High Yields From Small Samples
Consistent High Yields of Nuclei
See It In Action
Watch this video to learn how to prepare single-nuclei suspension from mouse kidney tissue on the Singulator 100 System.
Single Cells in 20-60 Minutes
Reproducibly generate suspensions of single cells in high yield with high-viability from a wide range of tissues.
Bright-field images of mouse kidney and spleen tissue cells.
High Cell Yields
High Cell Viabilities
Intuitive Software. Customizable Protocols.
Choose from a selection of pre-set protocols and pre-formulated reagents. Create your own protocols with customizable parameters, including mincing, enzyme incubation time, temperature, mixing and mechanical disruption profile. Optionally, use your own reagents.
1. Select from lists of Standard, Favorites, Recently Run protocols, or create your own protocol with customizable parameters.
2. Enter optional user notes and press RUN.
3. Step-by-step instructions and videos guide you through the system operation.
4. The internal camera and progress bar allow you to monitor tissue dissociation in real time.
Performance At A Glance
The Singulator 100TM System comes with a unique Single-Shot MechanismTM for convenient cell-isolation reagent loading and a Reagent Chiller that stores reagents for up to 100 nuclei runs.