Meridian Bioscience (Bioline)


Highly efficient purification of total RNA by combining the stringency of either guanidinium thiocyanate or guanidinium hydrochloride with the speed and purity of silica-membrane column purification.


RNA was isolated from 20 mg freeze-dried budding leaves of A. thaliana using ISOLATE II RNA Plant Kit. The extracted RNA was diluted in a 2-fold serial dilution (lanes 1-7) and PCR was performed using MyTaq One-Step RT-PCR Kit. The results illustrate the quality of the RNA obtained, as it can be used for very sensitive cDNA synthesis and PCR without further purification.

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