Delivering true single-cell spatial omics
Meet Curio Trekker—the first true single-cell mapping kit. Designed as a simple reagent kit, Curio Trekker integrates seamlessly with existing single-cell sequencing workflows and preserves the high molecular sensitivity of single-cell data.
With Curio Trekker, you can now transform standard single-cell genomics data into spatial data through a simple, straightforward workflow.
• One-of-a-kind spatial solution designed specifically for single-cell data
• Fits in front of any single-cell sequencing workflow
• Preserve the quality and sensitivity of single-cell data
• No deconvolution, no segmentation, no complex algorithms
• Simple 1-hour workflow with no specialized instrumentation
• Explore chromatin accessibility
Why stop at UMAP when you can reveal the spatial position of each single cell with a simple step using Curio Trekker?
Maximize your single-cell data with spatial positioning enabled by Curio Trekker.
Spatial mapping of nuclei from an adult mouse brain.
Adult mouse brain analyzed with Curio Trekker. Individual nuclei are color-coded by gene expression patterns from snRNA-seq data and spatially positioned, enabled by Curio Trekker tile. Specific features of the brain section and known cell types are highlighted.
The Curio Trekker protocol takes just one hour upstream of standard snRNA-seq workflows.
The workflow begins by melting a frozen tissue section on the Cruio Trekker tile, a spatially barcoded surface. Upon exposure to UV light, oligonucleotides carrying spatial barcodes are cleaved from the beads and attach to the nuclei in their vicinity. Spatially tagged nuclei are fed into standard single-nucleus RNA sequencing (snRNA-seq) workflows. Spatial barcode oligos are captured and amplified alongside cellular RNA. A custom bioinformatics pipeline is used to map the position of each nucleus based on the spatial barcodes it contains.
Spatial mapping of nuclei from an embryonic day 11 (E11) mouse embryo using the Curio Trekker workflow.
Mouse embryo (E11) analyzed with Curio Trekker. H&E-stained image and spatial map of the positioned nuclei display high concordance, demonstrating the accuracy of spatial feature preservation with Curio Trekker.
| Product Features | |
| Spatial resolution | True single-cell |
| Sample type | Fresh frozen tissues |
| Tile Size | 10 mm x 10 mm |
| Specialized capital equipment | None required |
| Required auxiliary equipment | Cryostat, single-cell sequencing platform, NGS sequencer |
| Workflow duration | 1 hour upstream of single-cell workflow |
| Sensitivity | Same as the molecule capture sensitivity of the single-cell workflow of choice |
| Single-cell workflow compatibility | |
Supported protocols
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User Demonstrated
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Ordering information
| Product Name | Part number |
| Trekker U 10×10 Bundle (4 Tiles) | SK017 |
| Trekker 10×10 Training Kit Bundle (2 Tiles) | SK020 |
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