Molecular IVD companies continue to experience tighter and tighter regulations in regard to incorporating appropriate positive and negative controls into their assays. Meridian’s qPCR Extraction Control Red uses E. coli cells of a known concentration that contain quantitative PCR positive control DNA sequence (with no known homology to any organism, so that it does not interfere with the detection of the sample target DNA). Their composition closely resembles a test sample and serves as a full-process quantitative PCR positive control, monitoring the success of a qPCR assay from lysis/extraction to amplification.
qPCR Extraction Control Red comprises internal control DNA sequence in E. coli cells and a control mix (containing specific primer and probe (emission wavelength = 670nm)) for qPCR detection. The cells are added to the sample and lysed and extracted with the sample DNA for downstream amplification by qPCR. The detection of qPCR Extraction Control acts as a PCR positive control and confirms the success of the extraction and amplification steps, avoiding the misinterpretation of a false negative result.
Control used to monitor the success of a qPCR assay from lysis/extraction to amplification and reduces the chance of obtaining false negative results from the sample DNA.
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