Air-Dryable™ Direct DNA qPCR Plant is a glycerol-free mix that contain optimized excipients compatible with air and oven drying. It has been designed for the development of qPCR assays for direct detection of plant DNA from crude lysate without extraction. Can also be used wet and/or with purified nucleic acid.
The introduction of genetically modified organisms (GMO) in the last 20 or so years and the demand for more precise and reliable techniques to detect foreign (transgenic or pathogenic) DNA in edible plants have been the driving force for the introduction of qPCR techniques in plant research. Direct amplification of crudely lysed plant DNA samples is a fast and convenient technique that avoids the need for laborious, time-consuming, and expensive nucleic acid extractions prior to qPCR. Air-Dryable™ Direct qPCR Plant is the first commercially available mix designed for developing ambient-temperature stable assays for the direct quantitation of DNA from plants. It combines the benefits of inhibitor tolerance with air-drying to create highly sensitive and cost-effective plant assays.
No need for extraction, compatible with 25% Plant lysate (SDS, NaOH). Perfect for multiplexing and fast cycling conditions for in-field qPCR testing. Used for genotyping and transgene or pathogenic detection.
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