Lyo-Ready Direct DNA qPCR and RNA/DNA qPCR Stool mixes are glycerol-free mixes that contain optimized excipients compatible with freezing and lyophilization and they are designed for the direct molecular quantitation of DNA and RNA from crudely processed stool specimens. Can also be used wet and/or with purified nucleic acid
PCR inhibitors found in stool specimens, such as bile salts, polysaccharides, hematin and catabolic substances, have posed a big challenge to developing assays that can directly amplify DNA or RNA. In addition, due to the high complexity and heterogeneity of fecal matter, sample preparation has traditionally been cumbersome, requiring the removal of possible interfering substances such as food debris, desquamated epithelial cells and mucus from the specimen. Lyo-Ready Direct DNA qPCR and RNA/DNA qPCR Stool mixes are designed for the direct qPCR/RT-qPCR analysis, requiring minimal stool sample processing. The mix contains an optimized blend of additives to negate inhibitors while maintaining the quality and integrity of the patient sample. As the need for fast, non-invasive testing for gastrointestinal conditions increases, short turn-around times, higher sensitivity and a long shelf-life become important distinguishing features.
If you are developing an infectious disease assay for parasites, viruses, bacteria, or a screening assay for inflammatory bowel disease or gastric or colon cancer, Lyo-Ready Direct DNA qPCR and RNA/DNA qPCR Stool mixes will speed up your assay development time. The mix only requires the addition of primers and probes to create a liquid or ambient-temperature stable assay that can be dried in a laboratory oven, avoiding the need for expensive lyophilization.
Inhibitor tolerance to 20% stool, 2 mg/mL bile salt and 40% Cary Blair media. Perfect for multiplexing and fast cycling conditions. Used in the detection of IBD, cancer, fecal microbiota and bacterial and viruses.
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