Lyo-Ready Direct DNA qPCR Blood, 4x

Blood is one of the most common specimens used for laboratory diagnostic testing and it is useful for evaluating the function of vital organs (kidneys, liver, thyroid, and heart) and for diagnosing diseases such as bacterial and viral infections, cancer, cardiovascular disease, and metabolic disorders such as diabetes. However, whole blood specimens, serum, and plasma contain a number of inherent PCR inhibitors including immunoglobulin G, hemoglobin, lactoferrin, and leukocyte DNA. In addition, PCR inhibitors can be found in the anticoagulants used to stabilize blood samples (e.g. EDTA, citrate, or heparin). Traditional methods have relied on removing these inhibitors by DNA or RNA extraction prior to testing, however, these methods are problematic, can cause sample loss, and are not 100% effective at removing all the inhibitors.

Lyo-Ready DNA qPCR Blood is a 4x qPCR master mix for the development of DNA blood tests, enabling highly sensitive detection of target DNA from whole blood, plasma, and serum samples. Lyo-Ready RNA/DNA qPCR Saliva is 4x qPCR master mix and can be used for the development of both RNA and DNA blood tests from whole blood, plasma, and serum samples. These mixes have been formulated specifically to overcome the inhibitors specifically found in blood samples – no further optimization is required aside from the addition of primers and probes. Furthermore, these mixes can be used in a wet format or lyophilized to create ambient-temperature stable whole blood tests, plasma tests or serum tests.