Molecular diagnostic tests are progressively moving towards lyophilized. There are several advantages for this, including room temperature shipping and storage, extended shelf-life and increased flexibility in sample volume. In order to be compatible with drying however, enzyme preparations must be glycerol-free and include specialized excipients that preserve the mixture as it is exposed to freezing, vacuum and dehydration. An ideal lyophilization formulation for example, should stabilize an enzyme in a freeze-dried format and allow very fast rehydration and reactivation of the enzyme preparations, without impacting its performance post rehydration. Can also be used wet and/or with purified nucleic acid
Lyo-Ready 1-Step RT-qPCR Mix is a glycerol-free formulation that contains Taq polymerase, reaction buffer, dNTP, MgCl2, lyo-excipients, and separate thermostable reverse transcriptase and dilution buffer. It is ideal for developing room temperature, lyophilized, multiplex RT-qPCR diagnostic tests and is suited for high-throughput, automated platforms requiring milli-second rehydration times.
Glycerol-free Taq polymerase, reaction buffer, dNTP, MgCl2, and lyo-excipients, and separate thermostable reverse transcriptase, ideal for developing room temperature, lyophilized, multiplex qPCR assays.
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