Fast qPCR master mix contains Taq polymerase, reaction buffer, dNTP, MgCl2, stabilizers, and PCR enhancers. It is ideal for developing multiplex qPCR diagnostic tests and is suited for high-throughput, automated platforms.
DNA target was amplified using standard qPCR cycling conditions (2 min @ 95ºC, 40 cycles of 10 sec @ 95ºC and 25 sec @ 60ºC) and the fast cycling conditions (2 min @ 95ºC, 40 cycles of 1 sec @ 95ºC and 10 sec @ 60ºC). The results illustrate the higher performance and end-fluorescence of the Fast qPCR Mix in both standard (blue) and fast (black) qPCR cycling conditions.
The latest advances in buffer chemistry and PCR enhancers and stabilizers, together with a hot-start polymerase, dNTPs and MgCl2, for highly reproducible, accurate assay results under fast thermal cycling conditions.
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