Clean Blood & Tissue DNA Kit

Genomic DNA analysis is the foundation of modern molecular diagnostics. Information from our genetic blueprint can provide answers to the cause and prevention of genetic diseases and cancer, but also offer insight into suitable treatment options. With the rise of personalised medicine, genomic DNA will be an important asset to track medicine response in a patient. We offer our Clean Blood & Tissue DNA Kit for the essential first step: extracting genomic DNA from blood or tissue.

The magnetic bead-based kit extracts genomic DNA from blood, tissue or buffy coat in four simple steps: lyse, bind, wash, and elute. Two different lysis buffers ensure optimal extraction from tissue, whereas blood and buffy coat only require one lysis step. With the binding solution and magnetic beads combined in one bottle, very efficient and robust workflows ensue.

Automation of the protocol is straightforward on our CleanXtract 96, and most other magnetic bead handlers or liquid handling devices.

Workflow

Samples are lysed using one of our specially formulated lysis buffers, which are optimized for the various types of starting material. Magnetic beads are added and DNA binds to them, after which the beads with DNA are separated from the lysate using a magnetic separation device. Rapid wash steps then ensure trace contaminants are removed, leaving the purified DNA to be eluted from the magnetic beads.

Proof of principle

To demonstrate the high DNA yield obtained from blood using the Clean Blood & Tissue DNA Kit Dx, genomic DNA was extracted on the CleanXtract 96 platform from 200 µL of human whole blood collected in EDTA tubes. Samples from 10 different donors were processed, with each donor extracted in triplicate. The same extractions were also performed manually using a kit from a Competitor. Higher DNA yields were obtained using the Clean Blood & Tissue DNA Kit Dx compared to the competitor kit.

FIGURE 1. DNA yield extracted from 10 blood samples of 200 µL from different donors, performed with the Clean Blood & Tissue DNA Kit versus a Competitor kit.

DNA purity was also analysed by measuring the OD values at 260/230 and 260/280. As Figure 2 clearly displays, the Clean Blood & Tissue DNA Kit Dx provides higher OD 260/230 values compared with the competitor kit, indicating higher DNA purity. Figure 3 shows that the 260/280 values of the DNA extracted with CleanNA’s kit are all higher than 1,80, indicating the DNA is free of proteins. Yield and OD values were measured on the DeNovix DS-11 instrument.

FIGURE 2. OD 260/230 of DNA extracted from 10 blood samples of 200 µL from different donors, performed with the Clean Blood & Tissue DNA Kit versus a Competitor kit.

FIGURE 3. OD 260/280 of DNA extracted from 10 blood samples of 200 µL from different donors, performed with the Clean Blood & Tissue DNA Kit versus a Competitor kit.

Additionally, a qPCR experiment was performed on the extracted DNA samples. In accordance with the yield measurements, the lower Ct values of the DNA extracted with the Clean Blood & Tissue DNA Kit Dx demonstrate that more target DNA is extracted compared to the competitor kit.

FIGURE 4. Albumin qPCR reaction results of DNA extracted from 10 blood samples of 200 µL from different donors, performed with the Clean Blood & Tissue DNA Kit versus a Competitor kit.