What is the difference between PCR and real-time PCR?

PCR measures the end-point, for example using an Agarose Gel. Nowadays people often use a quantification system like the DS-11, since this will give detailed information about the concentration and purity of your PCR product.

When performing a real-time (or qPCR) reaction, DNA can be measured during each cycle, allowing you to follow the amount of DNA present in your reaction tube.

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