Absorbance and Fluorescence Quantification

The DeNovix® DS-11 Series Spectrophotometer / Fluorometer enables precise absorbance and fluorescence quantification across a wide dynamic range. The dual mode spectrophotometer is equipped with SmartPath® Technology, which facilitates accurate and reproducible measurements for both cuvette and 1 μL absorbance modes. The proprietary optical core of the fluorescence component utilizes LED excitation sources and highly sensitive photodiodes capable of detecting minute amounts of fluorescence across four wavelength ranges.

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dsDNA Ultra-High Sensitivity Assay Performance Data

It can be a real challenge to measure very low concentrations of DNA and RNA. This application describes how concentrations ranging between 0.5 pg/µL to 300 pg/µL can be measured using the dsDNA Ultra High Sensitivity Assay from Denovix.

This assay is designed to measure dsDNA over RNA, ssDNA or proteins and can be used with fluorometers and fluorescence microplate readers.

To present the typical performance of the dsDNA Ultra High Sensitivity Assay, a dilution series of Calf thymus dsDNA is prepared in TE buffer. Three replicates of each samples, with concentrations between 0.5 pg/µL and 300 pg/µL, where measured on a DeNovix DS-11 FX fluorometer.

The obtained results show that the Assay enables accurate quantification of very low concentrations of dsDNA.

Click here for the application note to learn more about the dsDNA Ultra High Sensitivity Assay.

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NGS TruSeq Library Pooling

A major challenge within Next Generation Sequencing is an efficient library pooling. This application note describes how the Volume Verified Pipeting (VVP) 96 head in combination with FlexTrough can speed up your NGS TruSeq Library pooling.
The FlexTrough allows the use of a traditional 96 head, and decreases the amount of aspirate or dispense motions 1/10th the number of pipette motions, compared to an 8 tip system.
The combination of the FlexTrough and the VVP96 decreases the amount of motions even further, which makes the process up to 12 times faster compared to standard liquid handling with an 8-span.
Click here for the application note to learn more about the use of the VVP technology within the NGS Truseq library pooling.

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MyTaq DNA Polymerase – Colony PCR

The use of MyTaq polymerases in colony PCR is especially usefull because of the increased affinity for DNA. This increased affinity results in significant improvements with regards to yield, sensitivity and speed. The advantages are evident in nearly all templates, including complex genomic templates.
Optimization of colony PCR is traditionally done by purifying the host cell DNA template and followed by a conventional PCR. Within this PCR a thermostable DNA polymerase is used. While omitting the purification step brings numerous of advantages in the optimization, it is not very often possible to obtain good results without the purification. The Taq polymerase is the limiting factor in these crude samples.
This application note evaluates MyTaq and other similar polymerases in the optimization of colony PCR. Click here to find out how MyTaq performs in these experiments.

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Microvolume Nucleic Acid Performance Data

The Denovix DS-11 Microvolume spectrometers use the SmartPath technology. This technology allows for a higher reproducibility when measuring nucleic acid samples. Besides the ease-of-use, the accuracy, precision and reproducibility make the Denovix DS-11 instruments the ideal solution for microvolume spectrometry. Especially when measuring DNA and RNA concentrations.
The precision and accuracy of the SmartPath technology in the DS-11 spectrometers is highlighted by measuring a dilution series of double stranded DNA. The measurement is performed five times to emphasize the reproducibility of the technology. The obtained results in the experiment align with the criteria used on most molecular laboratories for nucleic acid measurements.
The complete data and experiment can be accessed here:

Seven Tips to Improve Cell Counting Accuracy

Cell Counting and viability measurements are important for a wide range of research applications across many diverse sample types.

This technical note describes seven top tips to ensure accurate and reproducible results when counting cells manually or using automated systems such as the CellDropTM Automated Cell Counter from DeNovix.

Click here for the application note:

CleanNGS – Purification for Next Generation Sequencing

With emerging NGS technologies, there is an increased need for NGS library purification methods providing accurate results starting from low input amounts of DNA and/or RNA. This application note will demonstrate the abilities of CleanNGS from CleanNA to provide both a high recovery as well as a high DNA and/or RNA purity. To demonstrate the purity of CleanNGS purified DNA, several qPCR experiment have been performed using sheared human genomic DNA.

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DNA Normalization

Time needed for liquid handling applications is mainly determined by the number of axis movements. Cutting down on the amount of axis movements is done by increasing the number of pipetting channels. The downside of increasing the number of pipetting channels is the loss of working with variable volumes in each channel.

An 8-span liquid handler does allow for independent volumes in an application, but comes with an increased amount of axis movements to obtain the same results.
The Volume Verified Pipetting (VVP) technology from Dynamic Devices, allows for the ideal combination between the two conventional setups. A 96 well head with each channel containing a unique volume and having its own sensor technology is now an option.

One of the easiest applications to imagine a use for this technique is the normalization of DNA in a High-Throughput application. Instead of doing 8 samples per transfer, and having to change tips in between, the VVP technology allows for a single step to transfer all 96 samples at once and without intermediate tip changes.

The application note elaborates on DNA normalization using the Volume Verified Pipetting technology.

Click here for the application note:

What is a high throughput assay?

An assay or application that is initially setup is often performed manually. This is very normal as there is a technician who is involved and little technical steps are performed in a very controlled environment (the technician’s eyes are on it). However, as the number of repeats increase or if the demand for the application increases a manual process is no longer the ideal solution. Automation is then the answer to make an application robust, reproducible and independent on technician skills. Automation is available in many forms (from a handheld repetition pipet up to a BioNex Hive platform). Once the step into automation is taken, the application can be considered to be of a higher throughput. The assay can vary and is not limited to rules. Any lab related process is able to be performed in high-throughput.

What is meant by high throughput?

There is no straight forward definition of high-throughput. The definition is depending on many aspects such as the complexity of the assay, the number of samples and also the used labware and volume range. For compound screening in the traditional pharmaceutical companies, screening is done on 384 or 1536 well plates. Running 100 plates containing 1536 samples, results in 153.600 individual data points, safe to say that this is high-throughput. To compare, a bloodbank may run two completely automated DNA extractions on large volume blood (3 ml) and only be able to run 72 or 96 samples per day. This is also considered to be high-throughput.

As there is not a straight forward definition, high-throughput is more of a term used for an increased amount of actions to be performed.