How does magnetic bead based isolation work?

Magnetic beads from CleanNA have a Polystyrene core, covered by a magnetic layer and a caboxylate-modified layer. This layer is negatively charged and binds water. By adding EtOH, DNA will attach to the water. Due to the magnet layer, beads can be drawned to a magnet. This will allow you to remove the supernatant and wash your solution. When using the CleanNA kits automated, we recommend to use a ring magnet.

When using the CleanNA kits, can I use my own lysis buffer?

We recommend to use the lysis buffer from CleanNA. All buffers in the CleanNA kits are designed to optimize the outcome of the kits. When changing one of the buffers, for example the lysis buffer, the results can be lower than expected.

What is the difference between magnetic bead based isolation and spin columns isolation?

The filter based method (also known as spin columns) has been the golden standard for Nucleic Acid isolation for a long time. Spin columns are easy to process, and prep time is relatively short. Yield and purity are consistent and high. So why use magnetic beads for your DNA or RNA isolation? Because spin columns are not easy to automate. There are a few manual steps involved when using spin columns that simply cannot be automated. When using magnetic beads, all steps can be automated using a liquid handler. You can either use a liquid handler already available on your lab, but you can also choose to go for a package-deal. The advantage of a package deal is that most suppliers will make sure that your protocols are pre-programmed on the liquid handler.

So what about the quality of my isolated DNA? Don’t worry about that! Compared to spin columns, DNA isolated with magnetic beads has the same quality.