The DeNovix® DS-11 Series Spectrophotometer / Fluorometer enables precise absorbance and fluorescence quantification across a wide dynamic range. The dual mode spectrophotometer is equipped with SmartPath® Technology, which facilitates accurate and reproducible measurements for both cuvette and 1 μL absorbance modes. The proprietary optical core of the fluorescence component utilizes LED excitation sources and highly sensitive photodiodes capable of detecting minute amounts of fluorescence across four wavelength ranges.
It can be a real challenge to measure very low concentrations of DNA and RNA. This application describes how concentrations ranging between 0.5 pg/µL to 300 pg/µL can be measured using the dsDNA Ultra High Sensitivity Assay from Denovix.
This assay is designed to measure dsDNA over RNA, ssDNA or proteins and can be used with fluorometers and fluorescence microplate readers.
To present the typical performance of the dsDNA Ultra High Sensitivity Assay, a dilution series of Calf thymus dsDNA is prepared in TE buffer. Three replicates of each samples, with concentrations between 0.5 pg/µL and 300 pg/µL, where measured on a DeNovix DS-11 FX fluorometer.
The obtained results show that the Assay enables accurate quantification of very low concentrations of dsDNA.
Click here for the application note to learn more about the dsDNA Ultra High Sensitivity Assay.
The Denovix DS-11 Microvolume spectrometers use the SmartPath technology. This technology allows for a higher reproducibility when measuring nucleic acid samples. Besides the ease-of-use, the accuracy, precision and reproducibility make the Denovix DS-11 instruments the ideal solution for microvolume spectrometry. Especially when measuring DNA and RNA concentrations.
The precision and accuracy of the SmartPath technology in the DS-11 spectrometers is highlighted by measuring a dilution series of double stranded DNA. The measurement is performed five times to emphasize the reproducibility of the technology. The obtained results in the experiment align with the criteria used on most molecular laboratories for nucleic acid measurements.
The complete data and experiment can be accessed here:
Cell Counting and viability measurements are important for a wide range of research applications across many diverse sample types.
This technical note describes seven top tips to ensure accurate and reproducible results when counting cells manually or using automated systems such as the CellDropTM Automated Cell Counter from DeNovix.
Click here for the application note:
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