MyTaq DNA Polymerase – Colony PCR

The use of MyTaq polymerases in colony PCR is especially usefull because of the increased affinity for DNA. This increased affinity results in significant improvements with regards to yield, sensitivity and speed. The advantages are evident in nearly all templates, including complex genomic templates.
Optimization of colony PCR is traditionally done by purifying the host cell DNA template and followed by a conventional PCR. Within this PCR a thermostable DNA polymerase is used. While omitting the purification step brings numerous of advantages in the optimization, it is not very often possible to obtain good results without the purification. The Taq polymerase is the limiting factor in these crude samples.
This application note evaluates MyTaq and other similar polymerases in the optimization of colony PCR. Click here to find out how MyTaq performs in these experiments.

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