Magnetic bead based isolation of viral DNA and viral RNA from nasopharyngeal SWABS and aspirates, as well as BAL samples
The Clean Viral DNA/RNA SP Kit is designed for reliable and high throughput isolation of viral DNA and viral RNA from nasopharyngeal SWABS, nasopharyngeal aspirates and bronchoalveolar lavage (BAL) samples. The kit allows for automated Coronavirus (SARS-CoV-2 / COVID-19) RNA isolation. CleanNA’s magnetic beads technology isolates high-quality nucleic acid that is free of proteins, nucleases, and other impurities.
The protocol is fully scalable and allows for Nucleic Acid isolation from various amounts of starting materials. Due to the use of our magnetic bead purification technology, the protocol can easily be automated on liquid handling workstations (e.g. Dynamic Devices LYNX™, Hamilton STAR™, Biomek™ i5, Biomek™ i7, Thermo KingFisher™ Flex, Applied Biosystems® MagMAX™, Qiagen BioSprint).
Our Clean Viral DNA & RNA SP Kit combines our propriety buffer system with the convenience of our CleanNA Particles to minimize the binding of PCR inhibiting compounds, present within the samples, onto our magnetic particles.
Using our specially formulated lysis buffer, samples are lysed and the nucleic acid is bound to our magnetic particles while DNases and RNases are deactivated. Nucleic Acid is isolated from the lysates in one step by binding to the CleanNA Particles’ surfaces. The CleanNA magnetic particles are separated from the lysates by using a magnetic separation device. Following a few rapid wash steps to remove trace contaminants (e.g. proteins and cellular debris), the purified DNA and RNA is eluted from the CleanNA particles for downstream applications using a nuclease-free water or a low ionic strength buffer.
The isolated nucleic acid is suitable for use in most downstream applications such as PCR, NGS and other enzymatic reactions.