Highly efficient purification of plasmid DNA by combining fast SDS/alkaline lysis of the starting material with the speed and purity of silica-membrane column purification.
• Fast – streamlined protocol for extraction of eighteen plasmid samples in as little as 15 minutes
• Efficient – rapid SDS/alkaline lysis and efficient column-binding for high-yield recovery of all plasmid molecules
• High-performance – efficient extraction of high-purity plasmid DNA, ideal for use in all downstream applications
• Flexible – reliable recovery of high- and low-copy plasmids from all gram-positive bacteria
The ISOLATE II Plasmid Mini Kit provides a simple, efficient column-based method for the isolation of plasmid DNA from bacterial cultures, without the need for hazardous reagents such as phenol.
By combining SDS/alkaline lysis with the speed and ease-of-use of silica-membrane purification, the ISOLATE II Plasmid Mini Kit provides a fast method for the purification of high-quality plasmid DNA. Separate protocols are provided for the isolation of high-copy plasmids, the isolation of low-copy plasmid, P1 constructs and cosmid DNA from E. coli and the isolation of plasmids from gram-positive bacteria such as Bacillus, Staphylococcus, Bifidobacteria or Corynebacteria.
The ISOLATE II Plasmid Mini Kit has been designed to deliver optimal performance in cloning, sequencing as well as end-point PCR alongside any enzyme from the Meridian PCR portfolio, including MyTaq DNA Polymerase. Additionally, the ISOLATE II Plasmid Mini Kit can be used in tandem with the SensiFAST Real-Time PCR Kits for high-performance qPCR.
| SKU: | Size: | Webshop: |
| BIO-52055 | 10 Preps | Order online |
| BIO-52056 | 50 Preps | Order online |
| BIO-52057 | 250 Preps | Order online |
All kit components should be stored dry and at room temperature. When stored under the recommended conditions and handled correctly, full activity of kit reagents is retained until the expiry date indicated on the outer box label.
Note: RNase A is supplied lyophilized. On addition of Resuspension Buffer P1, the RNase A solution should be kept stored at 4°C. When stored at 4°C. the RNase A solution is stable for at least 6 months.
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