Uracil DNA-Glycosilase (UDGase)

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Uracil DNA-Glycosilase (UDGase)

Uracil DNA Glycosylase (UDG) catalyzes the release of uracil from uracil-containing single-stranded or double-stranded DNA, but not from RNA or oligonucleotides (6 or fewer bases).

Product Highlights

  • Removes uracils from DNA – causes fragmentation of DNA and stops amplification in a PCR
  • Easy-to-use – novel, sensitive mutant is irreversibly inactive after heating

Product description

Uracil DNA Glycosylase (UDG) catalyzes the release of uracil from uracil-containing single-stranded or double-stranded DNA, but not from RNA or oligonucleotides (6 or fewer bases).

UDG is active over a broad pH range with an optimum at pH 8.0, does not require a divalent cation, and is inhibited by high ionic strength (>200 mM).

Meridian UDG is purified to SDS-PAGE purity and is free of endonucleases, exonucleases, nickases and RNases.

Source: Recombinant E. coli strain carrying the over expressed gene of Uracil DNA Glycosylase from E. coli.

Applications

  • Control of carry-over contamination in PCR
  • As a probe for protein-DNA interaction studies
  • Cloning of PCR products

SKU: BIO-27044
Size: 500 x 50µl Reactions 

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