Uracil DNA Glycosylase (UDG) catalyzes the release of uracil from uracil-containing single-stranded or double-stranded DNA, but not from RNA or oligonucleotides (6 or fewer bases).
Product Highlights
- Removes uracils from DNA – causes fragmentation of DNA and stops amplification in a PCR
- Easy-to-use – novel, sensitive mutant is irreversibly inactive after heating
Product description
Uracil DNA Glycosylase (UDG) catalyzes the release of uracil from uracil-containing single-stranded or double-stranded DNA, but not from RNA or oligonucleotides (6 or fewer bases).
UDG is active over a broad pH range with an optimum at pH 8.0, does not require a divalent cation, and is inhibited by high ionic strength (>200 mM).
Meridian UDG is purified to SDS-PAGE purity and is free of endonucleases, exonucleases, nickases and RNases.
Source: Recombinant E. coli strain carrying the over expressed gene of Uracil DNA Glycosylase from E. coli.
Applications
- Control of carry-over contamination in PCR
- As a probe for protein-DNA interaction studies
- Cloning of PCR products
| SKU: | Size: |
| BIO-27044 | 500 x 50µl Reactions |
