Clustered Regularly Interspaced Short Palindromic Repeats or CRISPR in combination with the cas9 enzyme are the basics for the CRISPR-cas9 technique allowing researcher to make changes to the genome. Even though the repetitive DNA sequences were first discovered in the 80’s in E. Coli, the real breakthrough for this technique the real breakthrough has been in 2007. Nowadays, the CRISPR-cas9 technique is used for many applications such as drug development, gene surgery, genetic variations, food and fuel research.