colonies on plate

GC biotech B.V.
Coenecoop 75
2741 PH Waddinxveen
The Netherlands

T : +31 (0) 182 22 33 00
F : +31 (0) 182 22 33 99

Trade Register : 27260248
ABN Amro : 4527.60.801
IBAN: NL52ABNA0452760801
V.A.T. : NL812123360B01

MyTaq DNA Polymerase

DNA amplification plays a critical role in many molecular biology procedures. Molecular analysis of thousands of genes and DNA templates is now routine, due to the advent of the PCR and the subsequent development of microarraying and high-throughput sequencing technology. For sequencing projects, the recombinant DNA template is normally purified from the host cell and then amplified by conventional PCR using a thermostable DNA polymerase. Alternatively, colony PCR can be performed by adding a single recombinant colony into a DNA polymerase PCR master mix, omitting the step of template purification. The use of this method however remains limited due to the inherent limitations of Taq DNA polymerase in crude sample PCR applications. Taq is easily inhibited by debris from bacterial cells and components of culture media, giving inconsistent results and only short fragments of cloned inserts can be interrogated.

MyTaq™ is a new generation of very high performance PCR products developed by Bioline, designed to deliver outstanding results on all templates, including complex genomic DNA templates. MyTaq is based on the latest technology in PCR enzyme preparation, engineered to increase affinity for DNA, so resulting in significant improvements to yield, sensitivity and speed. The enzyme is supplied with an industryleading novel buffer system, specifically formulated and validated for the unique properties of MyTaq.

In order to assess the suitability of MyTaq for colony PCR, the MyTaq Mix was compared with other similar polymerase mixes (see data below).